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. 2018 Jan 17;5(2):e437. doi: 10.1212/NXI.0000000000000437

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Copyright © 2018 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND), which permits downloading and sharing the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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(A) To determine whether the different tissue dissociation methods would yield a similar cellular composition of mononuclear cells from brain tissue, the percent of CD11b⁺, CD11c⁺, CD4⁺, CD8⁺, and CD19⁺ cells gated from live CD45⁺ cells, and (B) subsequently normalized to 100%. (C) The same mononuclear cell subsets from spinal cord were then gated from the live CD45⁺ population, and (D) normalized to 100%. The mean of a minimum of 4 separate tissue dissociations are shown.