Figure 6.

p62 overexpression is protective regarding cellular viability. Hep3B cells transiently transfected with Control plasmid or SQSTM1‐cherry were treated for 24 h with increasing concentrations of EFV, vehicle or thapsigargin (Tg) 2 μM. The efficacy of SQSTM1 overexpression was confirmed by WB (A). Quantitative analysis of the cell number (Hoechst fluorescence) by live cell fluorescence microscopy coupled with static cytometry (B). Data (mean ± SEM) were calculated as percentage of control (untreated Control cells). *P < 0.05, for efavirenz and #P < 0.05 for thapsigargin, significantly different from vehicle; one‐way ANOVA followed by a Newman–Keuls test. +P < 0.05, significantly different as indicated; Student's t‐test.