Gene Symbol	Sequence direction	Sequence	Band size (bp)	Annealing Tª (°C)
NLRP3	Sense	5′‐CTTCTCTGATGAGGCCCAAG‐3′	200	50
	Antisense	5′‐GCAGCAAACTGGAAAGGAAG‐3′
IL‐1β	Sense	5′‐TTCGACACATGGGATAACGAGG‐3′	84	52
	Antisense	5′‐TTTTTGCTGTGAGTCCCGGAG‐3′
IL‐6	Sense	5′‐CACTGGTCTTTTGGAGTTTGAGG‐3′	169	51
	Antisense	5′‐ ATTTGTGGTTGGGTCAGGGG‐3′
TNFα	Sense	5′‐AGCCGAATCGCCGTCTCCTA‐3′	124	58
	Antisense	5′‐CAGCGCTGAGTCGGTCACCC‐3′
SQSTM1	Sense	5′‐GGTTGCCTTTTCCAGTGACG‐3′	243	52
	Antisense	5′‐TCGCAGACGCTACACAAGTC‐3′
ACTB	Sense	5′‐AGCACGGCATCGTCACCAACT‐3′	183	58
	Antisense	5′‐ACATGGCTGGGGTGTTGAAGG −3′