Figure 4.

TUDCA improves intestinal barrier function. (A) The mRNA levels of ileum tight junction molecules were measured by real‐time PCR. (B, C) The mRNA levels of gut mucin lysozyme, Iap, Muc2 and C3gnt in the mice of different groups were detected by qPCR. (D) The TEERs of the CON, PA + LPS and PA + LPS + TUDCA groups were detected in Caco‐2 cells. CON, control group treated with vehicle; PA + LPS group, Caco‐2 cells co‐stimulated with 600 μM palmitate and 10 μg·mL⁻¹ LPS for the indicated times; PA + LPS + TUDCA group, Caco‐2 cells treated with the combination of palmitate (600 mΜ), LPS (10 μg·mL⁻¹) and TUDCA (500 mM) for the indicated times. The data are presented as the means ± SEM. One‐way ANOVA followed by the Newman–Keuls post hoc test for multiple comparisons. NCD group, n = 8; HFD group, n = 9; and HFD + TUDCA, n = 6. *P < 0.05; in vitro experiment, n = 5. *P < 0.05 versus the CON group; ^# P < 0.05 versus the PA + LPS group.