Fig. 5.

Production and characterization of recombinant cat flea proteins. Samples of recombinant proteins produced in E.coli were taken after purification by Ni affinity chromatography. Ten μg proteins were loaded per well in an SDS-12% polyacrylamide gel. The gel was stained with Coomassie Brilliant Blue (CB) or used for Western blot analysis (WB) using sera from vaccinated cats collected at T3. The position of the recombinant proteins is indicated with arrows. Some of the other protein bands in some of the samples correspond to E. coli contamination proteins, and aggregation or degradation products of the recombinant antigens (red dots). Abbreviations: MW, molecular weight markers (spectra multicolor broad range protein ladder; Thermo Scientific)