Table 2.
Cell-Type Distinguishing Genes Most Highly Elevated by RT
| 18w Fc vs noRT | 30w Fc vs noRT | ||||
|---|---|---|---|---|---|
| Gene ID | Description | RT | RT + FG | RT | RT + FG |
| Mast Cell | |||||
| Mcpt4 | mast cell protease 4 | 109.9 | 5.9 | 10.7 | 2.2 |
| Cpa3 | carboxypeptidase A3, mast cell | 94.2 | 3.3 | 19.9 | 3.0 |
| Cma2 | chymase 2, mast cell protease 9 | 51.6 | 3.6 | 9.0 | 2.0 |
| Cma1 | chymase 1, mast cell | 46.5 | 2.7 | 4.8 | 1.1 |
| Tpsg1 | tryptase gamma 1, mast cell protease 2 | 22.7 | 2.7 | 40.2 | 5.0 |
| Mrgprb1 | MAS-related GPR, member B1 | 20.3 | 2.9 | 8.5 | 1.7 |
| Darc | Duffy blood group, chemokine receptor | 18.5 | 1.9 | 2.3 | 0.9 |
| Tpsb2 | tryptase beta 2, mast cell protease 6 | 18.0 | 2.5 | 5.5 | 3.8 |
| Macrophage | |||||
| Mmp12 | matrix metallopeptidase 12 | 24.0 | 0.4 | 6.0 | 1.2 |
| Trem2 | triggering receptor expressed on myeloid cells 2 | 15.5 | 1.4 | 7.0 | 1.6 |
| Msr1 | macrophage scavenger receptor 1 | 10.0 | 0.5 | 5.1 | 1.2 |
| Cd200r1 | CD200 receptor 1 | 7.1 | 1.0 | 3.1 | 1.1 |
| Ccl3 | chemokine ligand 3 | 6.9 | 0.8 | 1.3 | 0.4 |
| Ms4a7 | membrane-spanning 4-domains, subfamily A, member 7 | 6.8 | 1.0 | 2.5 | 0.9 |
| Fcgr2b | Fc receptor, IgG, low affinity IIb | 6.6 | 0.7 | 2.2 | 1.3 |
| Ms4a6d | membrane-spanning 4-domains, subfamily A, member 6D | 6.5 | 0.4 | 2.4 | 1.2 |
| Dendritic Cell | |||||
| Cxcl9 | chemokine (C-X-C motif) ligand 9 | 79.8 | 4.0 | 1.1 | 0.5 |
| Kynu | kynureninase | 6.5 | 0.8 | 2.3 | 1.3 |
| Dnase1l3 | deoxyribonuclease 1-like 3 | 5.6 | 2.5 | 0.9 | 1.0 |
| Serpina3g | serine peptidase inhibitor, clade A, member 3G | 5.3 | 1.1 | 1.6 | 1.1 |
| Ppfia4 | protein tyrosine phosphatase, f polypeptide, interacting, a4 | 5.1 | 1.3 | 2.5 | 1.3 |
| Ffar2 | free fatty acid receptor 2 | 4.1 | 1.8 | 1.8 | 0.9 |
| Itgax | integrin alpha X | 4.1 | 0.7 | 1.9 | 0.8 |
| Ccl5 | chemokine (C-C motif) ligand 5 | 3.9 | 1.7 | 0.9 | 0.8 |
| Mesenchymal Cell | |||||
| Dlk1 | delta-like 1 homolog (Drosophila) | 15.2 | 4.7 | 26.3 | 10.4 |
| Timp1 | tissue inhibitor of metalloproteinase 1 | 8.2 | 0.4 | 3.6 | 0.8 |
| Fn1 | fibronectin 1 | 7.4 | 1.3 | 3.9 | 1.7 |
| Wisp1 | WNT1 inducible signaling pathway protein 1 | 5.3 | 0.9 | 2.4 | 1.5 |
| Ptx3 | pentraxin related gene | 4.7 | 1.1 | 1.6 | 1.2 |
| Col5a2 | collagen, type V, alpha 2 | 4.5 | 0.6 | 1.7 | 1.1 |
| Wisp2 | WNT1 inducible signaling pathway protein 2 | 4.0 | 0.9 | 2.7 | 1.3 |
| Fstl1 | follistatin-like 1 | 4.0 | 1.0 | 2.4 | 1.3 |
| Neutrophil | |||||
| Clec7a | C-type lectin domain family 7, member a | 6.0 | 0.7 | 1.5 | 0.6 |
| Aif1 | allograft inflammatory factor 1 | 4.1 | 1.1 | 2.0 | 1.1 |
| Ffar2 | free fatty acid receptor 2 | 4.1 | 1.8 | 1.8 | 0.9 |
| P2ry13 | purinergic receptor P2Y, G-protein coupled 13 | 2.7 | 0.4 | 1.3 | 0.9 |
| Fpr1 | formyl peptide receptor 1 | 2.4 | 0.7 | 1.6 | 0.8 |
| Lst1 | leukocyte specific transcript 1 | 2.3 | 0.8 | 1.1 | 0.8 |
| Mnda | myeloid cell nuclear differentiation antigen | 2.1 | 1.5 | 1.5 | 1.1 |
| B-cell | |||||
| Igl-V1 | immunoglobulin lambda chain (IgL) mRNA | 8.0 | 1.2 | 0.7 | 1.4 |
| March1 | membrane-associated ring finger (C3HC4) 1 | 3.3 | 1.1 | 1.7 | 1.3 |
| Ulbp1 | UL16 binding protein 1 | 3.3 | 0.7 | 1.8 | 1.3 |
| Cd22 | CD22 antigen | 3.0 | 2.4 | 1.3 | 1.3 |
| H2-DMb2 | histocompatibility 2, class II, locus Mb2 | 2.8 | 1.1 | 0.9 | 1.3 |
| Tlr1 | toll-like receptor 1 | 2.7 | 1.0 | 1.1 | 1.1 |
| Mef2c | myocyte enhancer factor 2C | 2.2 | 1.7 | 1.5 | 0.9 |
| Endothelial Cell | |||||
| Col4a1 | collagen, type IV, alpha 1 | 3.20 | 0.6 | 1.82 | 1.2 |
| Col4a2 | collagen, type IV, alpha 2 | 3.11 | 0.5 | 1.76 | 1.2 |
| Fabp4 | fatty acid binding protein 4, adipocyte | 3.01 | 1.2 | 1.73 | 0.5 |
| Mgp | matrix Gla protein | 2.07 | 0.7 | 1.39 | 1.1 |
| Multiple Potential Cell Sources | |||||
| Ankrd34b | ankyrin repeat domain 34B | 224.3 | 8.9 | 8.9 | 1.1 |
| Chl1 | cell adhesion molecule with homology to L1CAM | 93.8 | 1.3 | 15.6 | 1.0 |
| Ucma | upper zone of growth plate and cartilage matrix associated | 71.2 | 1.8 | 9.9 | 1.2 |
| Tnfsf18 | tumor necrosis factor (ligand) superfamily, member 18 | 46.8 | 3.6 | 2.8 | 1.0 |
| Gdf3 | growth differentiation factor 3 | 32.1 | 3.9 | 4.2 | 1.1 |
| Ear5 | eosinophil-associated, ribonuclease A family, member 5 | 29.5 | 2.1 | 18.3 | 1.6 |
| Ccl8 | chemokine (C-C motif) ligand 8 | 29.0 | 2.0 | 5.4 | 0.9 |
| Dio3 | deiodinase, iodothyronine type III | 25.6 | 1.4 | 10.3 | 1.1 |
Fold-change (Fc) values are relative to unirradiated (noRT) controls for RT alone and RT + FG-3019 (FG) treatment begun 16 weeks after RT