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. 2018 Jan 18;19:62. doi: 10.1186/s12864-018-4442-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Representative westernblots and in situ immunofluorescence of boar spermatozoa. Selected proteins were targeted with antibodies raised against human AQP-1 (45 kDa & 28/35 kDa), AQP-5 (28 kDa), AQP-7 (37 kDa), β-actin (43 kDa, the internal loading control), TF (80 kDa), PLG (90 kDa), FN-1 (220 kDa), TRXR-1 (55 kDa). In situ immunofluorescence micrographs of TRXT-1 and PLG are also shown. The FITC green fluorescence indicates the sub-apical (acrosome membrane) localization of TRXR-1 on the sperm head and the localization of PLG on the head and mid-piece of spermatozoon. Nuclei are counterstained in blue with DAPI