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. 2018 Jan 18;19:62. doi: 10.1186/s12864-018-4442-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Immunofluorescence quantification through flow cytometry. Columns labeled as “No” or “FITC” correspond to spermatozoa incubated without any antibodies or with FITC-conjugated secondary antibody only, respectively. a Percentage of immunopositive sperm cells and b Relative fluorescence intensity of labeled spermatozoa. Specific targeting is revealed above the blue line, suggesting for example for GLUT-5 detection, a lower proportion of cells being targeted (a) and which cells also show lowest or no specific detection of GLUT-5 (b)