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Expression and purification of the nucleocapsid protein of Tomato zonate spot virus. The recombinant TZSV N protein was expressed in Rosetta (DE3) and purified by Ni-NTA resin. The purified protein was separated on a 10% SDS-PAGE gel. The position of a 31-kDa recombinant N protein in the gel was indicated by an arrow. BSA and protein size marker were used as molecular weight reference
