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. 2017 Sep 26;12(1):51–57. doi: 10.1080/19336950.2017.1381805

Figure 1.

Figure 1.

Exon 33+/− cardiomyocyte has similar CaV1.2 channel properties with WT cardiomyocyte. (A) The stimulus waveform was used to induce ICa of cardiomyocyte, briefly ICa was recorded under the different testing potentials, increased from −60 mV to 60 mV (10 mV increase each step) followed by 10 mV depolarizing-pulse to induce steady-state inactivation in cardiomyocytes when using the 1.8 mmol/L Ca2+ as charger carrier. (B) Exemplary current traces were recorded from WT (black), exon 33+/− (blue) or exon 33−/− (red) cardiomyocytes. (C) Current densities of CaV1.2 channels in WT (n = 11 cells, V0.5 = 2.82±2.3 mV), exon 33+/− (n = 20 cells, V0.5 = 3.18±1.7 mV) and exon 33−/− (n = 18 cells, V0.5 = −7.28±3.3 mV) cardiomyocytes (*P<0.05 vs. WT, unpaired t test). (D) Normalized current-voltage relationships of CaV1.2 channels in WT, exon 33+/− and exon 33−/− cardiomyocytes. (E) Steady-state inactivation of CaV1.2 channels in WT (n = 16 cells, V0.5,inact = −20.63±0.44 mV), exon 33+/− (n = 14 cells, V0.5,inact = −20.46±0.85 mV) and exon 33−/− (n = 12 cells, V0.5,inact = −31.94±0.64 mV) cardiomyocytes.