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. 2001 Aug 21;1:11. doi: 10.1186/1471-2334-1-11

Table 1.

PCR Cycling Conditions

Cycle Conditions First Round1 Second Round1
Denaturation 94°C – 10 s 94°C – 10 s
Annealing 58°C – 10 s 67°C – 10 s
Extension 72°C – 30 s 72°C – 30 s
Number of Cycles 35 25

1The thermal cycler was held at 94°C for 3 min before cycling began to facilitate the transfer of specimens, held on ice, for a hot start procedure.