Effect of prenatal exposure to DEHP on serum sex steroid hormone levels at 1 year of age in the F1, F2, and F3 generations of mice. Sera were subjected to enzyme-linked immunosorbent assays for the measurements of (A) estradiol, (B) testosterone, and (C) progesterone. Graphs represent means ± standard error of the mean from 3 to 7 females per treatment group in the F1 generation, 3 to 10 females per treatment group in the F2 generation, and 3 to 7 females per treatment group in the F3 generation. *Significant differences compared with the control (P ≤ 0.05).