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. Author manuscript; available in PMC: 2019 Jan 16.
Published in final edited form as: Curr Protoc Mol Biol. 2018 Jan 16;121:31.13.1–31.13.18. doi: 10.1002/cpmb.50

Figure 2.

Figure 2

GM12878 LCL stably expressing activating KRAB-dCas9 was transduced with either control sgRNA or distinct sgRNAs targeting CD19 (CD19 sgRNA 1 and CD19 sgRNA 2). Two days after puromycin selection for cells that had been successfully transduced with sgRNA-encoding lentiviruses, cells were collected for flow cytometric analysis using antibodies against CD19.