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. Author manuscript; available in PMC: 2019 Jan 16.
Published in final edited form as: Curr Protoc Mol Biol. 2018 Jan 16;121:31.13.1–31.13.18. doi: 10.1002/cpmb.50

Figure 3.

Figure 3

(A) Genome snapshot displaying ChIP-seq and ChIA-PET tracks for various viral and host transcription factors at chr1: 38488884-38513387 (Consortium, 2012; Landt et al., 2012). The ChIA-PET RNAPII track indicates physical linkage between the intergenic enhancer and the POU3F1 promoter. (B) Quantitative RT-PCR of the POU3F1 transcript after CRISPRi treatment. GM12878 cells stably expressing KRAB-dCas9 were transduced with either sgCtrl (control non-targeting sgRNA) or one of three distinct sgRNAs targeting the intergenic enhancer between UTP11L and POU3F1 (sgPOU3F1). Total RNA was harvested and subject to quantitative RT-PCR. POU3F1 transcript levels were normalized to that of β-actin.