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. Author manuscript; available in PMC: 2019 Jan 16.
Published in final edited form as: Curr Protoc Mol Biol. 2018 Jan 16;121:31.13.1–31.13.18. doi: 10.1002/cpmb.50
CD19 genomic sequence: 5′……. ATAAACACACAAGATCATTTCCCGTGGTAG ...... 3′
3′……. TATTTGTGTGTTCTAGTAAAGGGCACCATC ……5′
CD19 sgRNA sequence: 5′-CACCGACACACAAGATCATTTCCCG -3′
 3′-CTGTGTGTTCTAGTAAAGGGCCAAA-5′