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. 2017 Dec 1;159(2):579–596. doi: 10.1210/en.2017-03061

Table 3.

Primers Used for qPCR

Gene Forward Primer Reverse Primer
Gapdh TGTCCGTCGTGGATCTGAC CCTGCTTCACCACCTTCTTG
Sf1 CGCTGTCCCTTCTGCGGCTT AGCACGCACAGCTTCCAGGC
Cyp11b1 AAGTATGGCCCCATTTACAGG CATTACCAAGGGGGTTGATG
Shh CCAATTACAACCCCGACATC GCATTTAACTTGTCTTTGCACCT
Gli1 CAGGGAAGAGAGCAGACTGAC CGCTGCTGCAAGAGGACT
Ctnnb1 GCAGCAGCAGTTTGTGGA TGTGGAGAGCTCCAGTACACC
Axin2 GCAGGAGCCTCACCCTTC TGCCAGTTTCTTTGGCTCTT
Lef1 CTGAAATCCCCACCTTCTACC TGGGATAAACAGGCTGACCT
Wnt4 CTGGACTCCCTCCCTGTCTT ATGCCCTTGTCACTGCAAA
Rspo3 TCAAAGGGAGAGCGAGGA CAGAGGAGGAGCTTGTTTCC