Figure 3.

Trabodenoson increased outflow facility in mouse eyes. Living mice were treated with 6% trabodenoson daily topically in one eye and placebo in the contralateral eye for two or seven consecutive days, with IOP measured daily. Immediately before perfusion experiments were initiated, mice were euthanized, eyes enucleated, and outflow facility measurements performed using an iPerfusion system. (A) Representative flow trace, comparing measurements in paired eyes in the presence or absence of trabodenoson at each pressure step (B). These data are plotted in (C), showing a flow-pressure relationship for this single experiment. Outflow facility was derived from each flow-pressure relationship and combined to determine the magnitude of trabodenoson effects. Outflow facility measurements for each experiment (indicated by number) and mean ± SD outflow facility (white line and gray shaded area, respectively) are displayed in “cello plots” as percentage change in trabodenoson treated compared to control for 2-day treatment of aged mouse eyes (D), 2-day treatment of all eyes (E), and 7-day treatment of all eyes (F). The data showed mean ± SEM, n = 9 (2 days aged), n = 17 (2 days young + aged) and n = 15 (7 days).