Figure 6.

Trabodenoson increases MMP-2 activity from TM cells. Three-dimensional human TM tissue constructs (3D-HTM) were treated with and 10 μM trabodenoson and the culture supernatants were analyzed at the 2- and 8-day time points. Secreted MMP-2 and TIMP-2 concentrations were measured using an electrochemiluminescence assay (A) and the activity of secreted MMP-2 from TM cells isolated from four donor on 3D-HTM constructs (TM1, TM2, TM3, TM4) was analyzed using gelatin zymography ([B, C]; n = 12, *P < 0.05, **P < 0.01).