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. 2018 Jan;59(1):383–392. doi: 10.1167/iovs.17-23212

Figure 7.

Figure 7

The effects of trabodenoson on MMP-14 and ECM proteins in TM cells. Three-dimensional human TM tissue constructs (3D-HTM) were treated with 1 and 10 μM trabodenoson and tissue constructs and their cell lysates were analyzed at the 2- and 8-day time points. After 2 days, treated 3D-HTM constructs were fixed and incubated with antibodies against collagen IV, and fibronectin and, in parallel, compared to 1 and 3 μg/mL MMP-2 treatments (A). Expression of active MMP-14, collagen IV, and fibronectin for 3D-HTM after 2- and 8-days of treatment was measured using Western blot. (B) Representative blots. (C) Densitometry analysis of all Western blot data (n = 12, *P < 0.05, **P < 0.01, ***P < 0.001).