Figure 2.

Efsevin Does Not Affect Beta-Adrenergic Stimulation in RyR2^R4496C/WT Cardiomyocytes

cAMP accumulation in freshly isolated RyR2^R4496C/WT cardiomyocytes was measured in response to different stimuli. Efsevin had no effect on cellular cAMP levels (p > 0.999, Kruskal-Wallis test), whereas ISO induced a significant increase in cellular cAMP (p < 0.001). This increase could be blocked using the beta-adrenoreceptor blocker propranolol (prop) (p = 0.036), although efsevin had no effect on the ISO-induced increase in cellular cAMP (p = 0.999), indicating that efsevin neither activates nor blocks the beta-adrenergic system in cardiomyocytes. ∗p < 0.05; ∗∗∗p < 0.001. Abbreviations as in Figure 1.