Table I.
Template | Primer sequence (5′→3′) | Annealing temperature (°C) | Cycle times | Product |
---|---|---|---|---|
APOEε3 | Forward: TGGAGGACGTGCGCGGCCGCCTGGTGCAG | 64 | 30 | APOE ε2 |
Reverse: TGTCCGCGCCCAGCCGGGCCTG | ||||
APOEε3 | Forward: TGACCTGCAGAAGTGCCTGGCAGTGTAC | 61 | 30 | APOE ε4 |
Reverse: TCGGCATCGCGGAGGAGCCGCTTA |
APOE, apolipoprotein E.