Figure 4.

Silencing of SOCS1 enhances the immunological effect of MUC1-CRT-primed 4T1-treated DCs in vivo. Mice were randomly divided into 5 groups (n=10 per group). Mice in each group were administered with an intradermal injection of 100 µl normal saline, 100 µl untreated DCs, 100 µl SOCS1 siRNA-treated DCs, 100 µl MUC1-CRT-primed 4T1-treated DCs or 100 µl SOCS1 siRNA- and MUC1-CRT-primed 4T1-treated (double-treated) DCs. (A) Example images demonstrate tumor sizes. (B) Tumor volume (mm³) was assessed every other day. Tumor sizes are presented as (C) an image, (D) tumor weight and (E) tumor volume following sacrifice and tumor isolation 16 days after treatment. Secretion of the cytokines, (F) IFN-γ and (G) TNF-α. *P<0.05, **P<0.01 and ***P<0.001 vs. NC; ^#P<0.05, ^##P<0.01. SOCS1, suppressor of cytokine signaling 1; siRNA, small interfering RNA; MUC1, type I transmembrane glycoprotein mucin 1; CRT, calreticulin; DCs, dendritic cells; IFN-γ, interferon-γ; TNF-α, tumor necrosis factor-α.