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In vitro anticancer activities of 2 µM bufotalin and 4 µM bufalin. (A) Chemical structure of bufotalin and bufalin. (B) Cytotoxicity of bufotalin and bufalin towards human cancer and normal cells. The ESCC cells and Het-1A human esophageal squamous cells were treated with bufotalin and bufalin for 72 h, and the cell viability was examined by MTT assay.
