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Apoptotic DNA fragmentation and nuclear condensation induced by bufotalin, examined using a TUNEL and DAPI co-staining assay. The cells were treated with bufotalin for 24 h. Green fluorescence indicates apoptotic DNA fragmentation, while the arrows in the DAPI-staining images indicate the nuclear condensation. Values at the bottom of the images indicate the percentage of apoptotic cells. Magnification, ×200. TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.
