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Activation of p53 in bufotalin-induced apoptosis. (A) Phosphorylation of p53 at Ser15 and expression of total p53 in Eca-109 cells treated with bufotalin at 2 and 4 µM for 24 h, assessed by western blotting. Quantitative analysis was performed using Quantity-one 1D software. (B) Immunofluorescence of p-p53 in cells treated with bufotalin for 24 h. p53, tumor protein p53; p-, phosphorylated.
