Skip to main content
. 2017 Nov 23;15(2):1360–1366. doi: 10.3892/etm.2017.5558

Figure 2.

Figure 2.

Knockdown of Nek7 in Y79 and SO-RB50 cells by lentivirus-delivered shNek7-1 and shNek7-2. (A) Detection of lentiviral transfection efficiency. Y79 and SO-RB50 cells were transfected with shNC, shNek7-1 or shNek-2, and bright field (top) or green fluorescence protein-staining (bottom) images were obtained at 96 h after transfection. Scale bar, 100 µm and magnification, ×200. (B) Nek7 mRNA expression and (C) Nek7 protein expression determined by RT-qPCR and western blot analysis, respectively, in Y79 cells transfected with shNC, shNek7-1 and shNek7-2. (D) Nek7 mRNA expression and (E) Nek7 protein expression determined by RT-qPCR and western blot analysis, respectively, in SO-RB50 cells transfected with shNC, shNek7-1 and shNek7-2. Experiments were repeated three times. **P<0.01 and ***P<0.001 vs. shNC group. Nek7, NIMA-related kinase-7; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; shNC, negative control short hairpin RNA.