Figure 5.

Permeability assay and Transwell migration assay. (A) GBM-ECs were pretreated with LY294002 inhibitor or DMSO as control. Then, the cells were grown to confluence on Transwell filters. Permeability for fluorescein isothiocyanate-dextran (70 kDa) was measured using a fluorescence plate reader (λ excitation 485 nm; λ emission 530 nm). (B) Inhibitor or DMSO (control)-treated GBM-ECs were plated onto the upper chamber of an 8-µm Transwell system. Cells were allowed to migrate for 4 h. Migrated cells were quantified by counting crystal violet-stained cells in 4 random fields/filter. Results are presented as the mean ± standard error of 3 independent experiments. *P<0.05 vs. negative control. GBM-ECs, glioblastoma endothelial cells; DMSO, dimethylsulfoxide.