miR-152 suppresses EOC cell proliferation and migration in vitro. (A) miR-152 was overexpressed in EOC cell lines, SKOV3 and OVCAR3, by transfection with miR-152 mimic, or cells were transfected with miR-ctrl. The transfection efficiency was confirmed by reverse transcription-quantitative polymerase chain reaction. Data are presented as the mean + SD. MTT proliferation assay was used to detect the growth ability of (B) SKOV3 and (C) OVCAR3 cells transfected with miR-152 mimic or miR-ctrl. Data are presented as the mean ± SD. (D) Transwell assay was used to detect the migration of OVCAR3 cells transfected with miR-152 mimic or miR-ctrl. In total, 48 h after transfection, the cells were allowed to invade toward the lower chamber and the relative photos were captured under a microscope at ×20 magnification (stain, crystal violet). Data are presented as the mean + SD. Each result represents an average of triplicates. *P<0.05 and **P<0.01 vs. miR-ctrl. miR, microRNA; EOC, epithelial ovarian cancer; ctrl, control; SD, standard deviation; OD, optical density.