Figure 5.

Combination treatment of rapamycin and COE could further give the raise to the DNA damage and increase the amount of apoptosis bodies. ECA-109 cells were treated with the solution of different ingredients (COE 80 mg/l, rapamycin 100 nM) for 24 h. (A) DNA damage response was performed with terminal deoxynucleotidyl transferase dUTP nick end labeling assay and quantified under a microscope (magnification, ×400). The red arrow indicates the representative DNA damage. **P<0.01 vs. control. (B) The apoptotic bodies (arrows) were more numerous with combination treatment, as assessed using transmission electron microscopy. Scale bar, 0.5 µm. COE, Celastrus orbiculatus extracts.