Figure 3.

BDNF is a direct target of miR-613 in GC cells. (A) miR-613 binding sites in the BDNF 3′UTR. Mut BDNF indicates the BDNF 3′UTR with a mutation in the miR-613 binding site. (B) Luciferase assay in SGC-7901 cells co-transfected with miR-613 mimic or miR-NC and luciferase reporter plasmid (WT/Mut 3′UTR BDNF). (C and D) BDNF expression in SGC-7901 cells after transfection with miR-613 mimics or miR-NC was determined at (C) the mRNA level by reverse-transcription quantitative polymerase chain reaction and (D) the protein level by western blot analysis. β-actin was used as an internal control. **P<0.01. WT, wild-type; Mut, mutant-type; UTR, untranslated region; miR-NC, negative control microRNA; BDNF, brain-derived neurotrophic factor.