Figure 4.

Enhanced autophagy of glioblastoma cells by bevacizumab treatment. (A) U87-MG cells were treated with different concentrations of bevacizumab for 48 h, and then the cells were lysed for western blot analysis for indicative hallmarks of autophagy. GAPDH was used as the loading control. The result was a representative of three independent experiments. (B) The quantification of LC3B-II/LC3B-I ratio and SQSTM1(p62)/GAPDH ratio for result in A, both were taken as hallmarks to assess the autophagy level. Error bars represented mean ± SD. P-values were determined by the one-way analysis of variance (ANOVA) followed by Tukeys post hoc test. ***P<0.001, **P<0.01.