Figure 5.

Bevacizumab can suppress the Akt-mTOR signaling pathway of glioblastoma cells to enhance their autophagy. (A) U87-MG cells were treated with different concentrations of bevacizumab for 48 h, and then the cells were lysed for western blot analysis to assess the phosphorylation of indicative hallmarks in Akt-mTOR signaling pathway that involved in autophagy. GAPDH was used as the loading control. The result was a representative of three independent experiments. (B) The quantification of phosphorylated Akt (S473), Akt (T308), phosphorylated mTOR (S2448) and phosphorylated p70S6K those normalized to their corresponding total proteins, respectively, for result in A, all of them can be used to reflect the mTOR activity. Error bars represented mean ± SD. P-values were determined by the one-way ANOVA followed by Tukey post hoc test. ***P<0.001, **P<0.01. *P<0.05. ANOVA, analysis of variance.