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. 2017 Nov 21;15(2):1607–1615. doi: 10.3892/ol.2017.7454

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Copyright: © Hu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Sera from high-fat diet-fed TRAMP mice promoted proliferation, migration and invasion of prostate cancer cells. (A) DU145 cells were treated with SFM, CM containing 2.5% mixed sera from CD-fed TRAMP mice or CM containing 2.5% mixed sera from HFD-fed TRAMP mice. Cell viability was determined using a Cell Counting Kit-8 assay. (B) Effects of SFM, CD-CM and HFD-CM culture on the migration of DU145 cells as determined using a wound-healing assay. (C) Representative images of the wound-healing experiment demonstrating the migration of DU145 cells cultured with SFM, CD-CM and HFD-CM, respectively. (D) Invasion of DU145 cells was determined using a Matrigel-Transwell invasion assay. SFM, CD-sera-CM or HFD-sera-CM was added to the lower chamber of the Transwell as the chemoattractant. (E) Representative images of the Matrigel-Transwell experiment, demonstrating the invasive abilities of DU145 cells cultured with SFM, CD-CM and HFD-CM, respectively. Results are presented as the mean ± standard error of the mean (n=3). *P<0.05, **P<0.01 and ***P<0.001. SFM, serum-free medium; CD, control diet; HFD, high-fat diet; CM, conditioned media; TRAMP, transgenic adenocarcinoma of mouse prostate.