Figure 1.

Luteolin attenuates cytosolic calcium accumulation. Multiphoton microscopy was applied to detect cytosolic Ca²⁺ in cardiomyocytes preserved in the following solutions: (A) UW solution; (B) DMEM; (C) 7.5 µmol/l luteolin supplemented UW solution; (D) 15 µmol/l luteolin supplemented UW solution and (E) 30 µmol/l luteolin supplemented UW solution. Scale bar, 50 µm. Green fluorescence indicates Fluro-3 labeled Ca²⁺, while blue fluorescence indicates stained nuclei. UW solution, University of Wisconsin solution; DMEM, Dulbecco's modified Eagle's medium.