Figure 5.

Luteolin suppresses calcium overload in the SR. An SR calcium kit was used to detect Ca²⁺ influx in the SR in myocytes preserved in the following solutions: (A) UW solution; (B) DMEM; (C) 7.5 µmol/l luteolin supplemented UW solution; (D) 15 µmol/l luteolin supplemented UW solution; and (E) 30 µmol/l luteolin supplemented UW solution. Representative multiphoton microscopy images from indicated time points were captured over the hypothermic preservation period. Scale bar, 50 µm. Green fluorescence indicates fluo-3 labeled Ca²⁺, while blue fluorescence indicates stained nuclei. UW solution, University of Wisconsin solution; DMEM, Dulbecco's modified Eagle's medium; SR, sarcoplasmic reticulum.