Figure 4.

Human macrophages phagocytize esophageal squamous cancer cells. (A) pHrodo Red-labeled esophageal cancer cells were incubated with human M2 macrophages and the indicated antibodies and examined by immunofluorescence microscopy to detect phagocytosis. Photomicrographs from the representative samples are presented (magnification, ×200 and ×400). (B) Phagocytic activity was assessed by determining the phagocytic index following incubation with the indicated antibodies. (C) Phagocytic activity was assessed by determining the phagocytic index following incubation with dose-titrated indicated antibodies. *P<0.05 and ^#P<0.01.