Figure 1.

Phenotyping Pipeline for GWA Mapping.

Aphid feeding behavior was quantified on 350 natural Arabidopsis accessions with a video-tracking platform. Each aphid was released in an enclosed well of a 96-well plate, containing a leaf disc embedded on agar. With a top-view camera and video-tracking software, aphid position (on leaf disc or wall) and velocity were recorded and used for the estimation of the number and duration of probes (Kloth et al., 2015). Probes were classified into short probes (<3 min) that were most likely associated with superficial cell layers, and long probes (>25 min) that were potentially related to phloem feeding. Aphid probing parameters were mapped to 214 k polymorphisms on the Arabidopsis genome to identify genes involved in plant resistance to aphids.