Table 1. Aphid Feeding Behavior, Measured by 8-h Electrical Penetration Graph Recordings on the Natural Arabidopsis Accessions Col-0 and C24, a NIL of Col-0 with a C24 Introgression Covering the Region around SLI1, and the T-DNA Lines sli1-1 and sli1-2.

Variable	20°C					26°C
	Col-0	C24	NIL	sli1-1	sli1-2	Col-0	C24	NIL	sli1-1	sli1-2
Total duration nonprobing	196 ± 30	118 ± 18*	169 ± 27ns	164 ± 15ns	107 ± 22*	141 ± 14	98.3 ± 13*	129 ± 18ns	55.2 ± 12***	119 ± 24ns
Total duration epidermis/mesophyll	150 ± 21	169 ± 24ns	154 ± 16ns	121 ± 15ns	91.5 ± 25ns	181 ± 13	176 ± 16ns	152 ± 19ns	69.7 ± 9***	93.9 ± 16**
Number of probes <3 min	26.8 ± 4.4	23.6 ± 3.5ns	29.8 ± 5.2ns	28.5 ± 4.2ns	26.2 ± 9.2ns	27.4 ± 3.9	29.9 ± 5ns	27.1 ± 4.2ns	8.4 ± 2.2***	21.5 ± 5.6ns
Total duration difficulties	0 ± 0	0 ± 0ns	0 ± 0ns	18.3 ± 8.5*	0 ± 0ns	9.5 ± 6.9	2.6 ± 2ns	3.2 ± 3.2ns	1.4 ± 1.4ns	11.8 ± 5.7ns
Number of difficulties	0 ± 0	0 ± 0ns	0 ± 0ns	0.8 ± 0.3*	0 ± 0ns	0.2 ± 0.1	0.1 ± 0.1ns	0.1 ± 0.1ns	0 ± 0ns	0.8 ± 0.4ns
Latency to 1st salivation	156 ± 27	128 ± 17ns	122 ± 18ns	166 ± 26ns	128 ± 22ns	116 ± 22	83.4 ± 14ns	99 ± 23ns	102 ± 17ns	179 ± 36ns
Total duration salivation	6.4 ± 1.6	5.6 ± 0.8ns	6.1 ± 1.2ns	3.9 ± 0.7ns	1.4 ± 0.3*	9.4 ± 2.3	6.1 ± 0.9ns	4.5 ± 0.9*	1.8 ± 0.5***	1.3 ± 0.3***
Number of salivations	6.5 ± 1.2	8.8 ± 1.3ns	8.6 ± 1.4ns	5.4 ± 1ns	1.8 ± 0.3*	9.2 ± 0.9	11.1 ± 1.4*	7.1 ± 0.9ns	2.2 ± 0.3***	1.7 ± 0.4***
Number of single salivations	0.9 ± 0.4	0.2 ± 0.1ns	0.5 ± 0.2ns	0.2 ± 0.1ns	0.2 ± 0.1ns	0.6 ± 0.3	0.2 ± 0.2ns	0.5 ± 0.2ns	0.3 ± 0.1ns	0.1 ± 0.1*
Mean duration salivation	1 ± 0.2	0.7 ± 0.1ns	0.7 ± 0.1ns	0.8 ± 0.1ns	0.8 ± 0.1ns	1 ± 0.2	0.6 ± 0.1*	0.6 ± 0.1*	0.7 ± 0.1ns	0.8 ± 0.1ns
% Salivation in phloem phase	11.9 ± 4.9	4.3 ± 0.8ns	8.2 ± 2.4ns	3.1 ± 0.8ns	2 ± 1.5**	8.5 ± 1.5	3.6 ± 0.6*	5.8 ± 2ns	0.6 ± 0.2***	4.2 ± 2.4**
Latency to 1st phloem feeding	161 ± 27	138 ± 19ns	123 ± 18ns	167 ± 26ns	138 ± 28ns	129 ± 23	83.9 ± 14ns	114 ± 24ns	110 ± 19ns	186 ± 39ns
Total duration phloem feeding	118 ± 34	187 ± 34ns	151 ± 36ns	173 ± 20ns	280 ± 41*	137 ± 20	198 ± 23*	192 ± 35ns	345 ± 18***	251.1 ± 41*
Total duration sustained feeding	107 ± 34	169 ± 35ns	132 ± 37ns	166 ± 21*	279 ± 41*	119 ± 21	170 ± 25ns	174 ± 36ns	344 ± 18***	249 ± 42*
Number of phloem feedings	5.2 ± 1	8.2 ± 1.2ns	7.3 ± 1.3ns	4.9 ± 0.9ns	1.7 ± 0.3*	7 ± 0.9	10 ± 1.2ns	6.3 ± 1ns	1.8 ± 0.2***	1.5 ± 0.3***
Number of sustained feedings	2.2 ± 2.2	4.4 ± 4.4**	2.7 ± 2.7ns	2.8 ± 2.8ns	1.2 ± 1.2ns	2.8 ± 2.8	4.3 ± 4.3*	2.3 ± 2.3ns	1.4 ± 1.4***	0.9 ± 0.9***
Mean duration phloem feeding	54.7 ± 38	33.8 ± 12ns	30 ± 11ns	62.6 ± 13ns	232 ± 44**	26 ± 5.7	39.5 ± 17ns	45.2 ± 16ns	254 ± 31***	222 ± 42**
Mean duration sustained feeding	78 ± 40	42.2 ± 11ns	47.8 ± 12ns	108 ± 25ns	270 ± 36***	50.6 ± 10	59 ± 18ns	85.5 ± 17*	298 ± 26***	273 ± 39***
% Aphids phloem feeding	84.6	100 ± 0ns	100 ± 0ns	100	92.3	100	100	100	100	93.8
Total duration xylem ingestion	9.8 ± 5.8	0 ± 0*	0 ± 0*	0 ± 0*	0.5 ± 0.5ns	1.9 ± 1.7	0 ± 0ns	0 ± 0ns	6.6 ± 3.1ns	2.8 ± 2.8ns
Number of xylem ingestions	0.5 ± 0.2	0 ± 0*	0 ± 0*	0 ± 0*	0.1 ± 0.1ns	0.1 ± 0.1	0 ± 0ns	0 ± 0ns	0.2 ± 0.1ns	0.1 ± 0.1ns

Time is shown in minutes. Mean ± se. Mann-Whitney U pairwise comparisons were performed between Col-0 and the plant lines C24, NIL, sli1-1, and sli1-2 for temperature treatments separately (*P < 0.05, **P < 0.01, and ***P < 0.001). Significant differences between 20 and 26°C within plant lines are shown in bold (Mann-Whitney U pairwise comparisons, P < 0.05). “Difficulties” involve penetration difficulties. “Sustained” involves events > 10 min. Latency was measured from the start of the recording. Single salivations were phloem salivations that were not directly followed or preceded by phloem ingestion. Between 12 and 20 biological replicates were measured (20°C: Col-0 n = 13, C24 n = 12, NIL n = 12, sli1-1 n = 16, sli1-2 n = 13; 26°C: Col-0 n = 20, C24 n = 14, NIL n = 14, sli1-1 n = 20, sli1-2 n = 16).