Figure 5.

Nitrate and Chloride Transport Activity of Maize NPF6.4:Y370H.

(A) Kinetic analysis of NPF6.4:Y370H nitrate transport activity. Net nitrate uptake values of NPF6.4:Y370H-injected oocytes from 25 μM to 20 mM were fitted by the Michaelis-Menten equation. Uptake values at low concentrations are shown in the figure inset. n = 7 oocytes.

(B) Kinetic analysis of NPF6.4:Y370H chloride transport activity. Net chloride uptake values of NPF6.4:Y370H-injected oocytes from 50 μM to 1 mM were fitted by a linear regression analysis. n = 7 oocytes.

Data represent net substrate uptake values with water-injected control values subtracted. Values indicate means ± se.