Figure 1.

CAMTA3-GFP Represses Expression of SA Pathway Genes in camta2 camta3 Plants.

(A) The CAMTA3p:CAMTA3-GFP construct was transformed into camta2 camta3 mutant plants and three transgenic lines—C3, C5, and C23—were characterized. Plants were grown for 20 d at 22°C under a 12-h photoperiod and relative transcript levels were determined by RT-qPCR. In the immunoblot analysis, anti-GFP antibody was used to detect the CAMTA3-GFP protein and antihistone H3 antibody was used to detect histone H3, which served as the loading control. Genes used for normalization for RT-qPCR are indicated in Methods, and data were subjected to ANOVA as detailed in Methods. Error bars indicate se (n = 3 biological replicates). Bars marked with different letters are significantly different (LSD, P < 0.05)

(B) Transcript levels of PR1, ICS1, CBP60g, and SARD1 were determined in the transgenic lines C3, C5, and C23 grown under the same conditions as in (A). Data were subjected to ANOVA as detailed in Methods. Error bars indicate se (n = 3 biological replicates). Bars marked with different letters are significantly different (LSD, P < 0.05)

(C) Photographs of wild-type, camta2 camta3, and transgenic plants after growth for 32 and 49 d at 22°C under a 12-h photoperiod.