Table 2. Gas Exchange of PGLP1 Antisense and Overexpressor Lines.
| Parameter | pglp1 | A1 | A8 | A2 | Col-0 |
|---|---|---|---|---|---|
| gs HC | 0.08 ± 0.02 | 0.07 ± 0.02 | 0.20 ± 0.01 | 0.12 ± 0.03 | 0.12 ± 0.01 |
| gs 1 d LC | 0.05 ± 0.01 | 0.06 ± 0.01 | 0.06 ± 0.01 | 0.07 ± 0.01 | 0.12 ± 0.02 |
| E HC | 1.63 ± 0.40 | 1.09 ± 0.22 | 2.56 ± 0.09 | 1.65 ± 0.23 | 1.63 ± 0.13 |
| E 1 d LC | 0.83 ± 0.18 | 1.08 ± 0.23 | 1.12 ± 0.20 | 1.34 ± 0.23 | 1.87 ± 0.18 |
| O1 |
O9 |
O8 |
Col-0 |
||
| gs10 | 0.22 ± 0.07 | 0.22 ± 0.05 | 0.24 ± 0.03 | 0.18 ± 0.04 | |
| gs21 | 0.30 ± 0.05 | 0.24 ± 0.03 | 0.27 ± 0.06 | 0.26 ± 0.04 | |
| gs40 | 0.23 ± 0.06 | 0.26 ± 0.03 | 0.23 ± 0.03 | 0.15 ± 0.03 | |
| A10 | 13.54 ± 1.14 | 13.74 ± 2.06 | 14.36 ± 0.66 | 13.56 ± 0.84 | |
| A21 | 12.09 ± 1.87 | 12.26 ± 1.17 | 12.32 ± 1.45 | 11.35 ± 1.52 | |
| A40 | 8.33 ± 0.55 | 7.46 ± 0.68 | 8.10 ± 1.69 | 6.20 ± 0.58 | |
| O2 inhibition | 30.28 ± 7.31 | 38.85 ± 6.49 | 43.27 ± 9.88 | 44.98 ± 5.26 | |
| Γ10 | 26.0 ± 4.50 | 24.2 ± 4.92 | 21.8 ± 5.07 | 24.6 ± 3.68 | |
| Γ21 | 57.9 ± 10.3 | 62.2 ± 9.04 | 55.9 ± 5.55 | 62.0 ± 3.54 | |
| Γ40 | 104.1 ± 4.48 | 118.8 ± 8.57 | 114.4 ± 12.2 | 111.0 ± 3.29 | |
| Γ21* | 36.6 ± 1.54 | 27.9 ± 4.66 | 28.1 ± 4.38 | 32.9 ± 3.93 | |
| Rn | 1.03 ± 0.10 | 1.07 ± 0.20 | 1.08 ± 0.16 | 0.84 ± 0.12 | |
| Rd | 1.21 ± 0.08 | 1.33 ± 0.18 | 1.27 ± 0.29 | 1.36 ± 0.24 | |
| Rp | 1.76 ± 0.24 | 1.54 ± 0.37 | 1.45 ± 0.17 | 1.38 ± 0.08 |
Antisense and overexpressor plants were grown in HC and LC, respectively, under conditions as in Figure 1D. Stomatal conductance and transpiration of antisense plants were measured before and at day 1 after transfer from HC to LC. Values are means ± sd (at least four different plants per genotype). Numbers in bold are significantly different from the wild-type value based on Student’s t test (P < 0.05). Units are mol H2O m−2 s−1 (gs), µmol CO2 m−2 s−1 (A, Rd, Rn, and Rp), µL L−1 (Γ and Γ21*), and mmol H2O m−2 s−1 (E). O2 inhibition of A was calculated as 100(A21 − A40)/A21. Values for the pglp1 mutant are included for comparison from a previous study (Timm et al., 2012b). A, net CO2 uptake; Γ, CO2 compensation point; Γ21*, Γ21 corrected for day respiration; gs, stomatal conductance; Rd, daytime respiration; Rn, nighttime respiration; Rp, photorespiration; indices are 10, 21, and 40% O2.