ZYGO1 Promotes Homologous Pairing Independent of HEI10 and OsZIP4, but Affects HEI10 Distribution.
(A) Chromosome behavior of meiocytes in hei10, Oszip4, hei10 zygo1, and Oszip4 zygo1 at pachytene or late post-leptotene stage. Full-length chromosome pairing in hei10 and Oszip4 is suppressed in hei10 zygo1 and Oszip4 zygo1 double mutants. Chromosomes were stained with DAPI.
(B) Triple immunolocalization of OsREC8 (red), HEI10 (green), and ZEP1 (blue) in the wild type and zygo1 at late pachytene or late post-leptotene stage. The bright punctuated signals of HEI10 are restricted to the short ZEP1 stretches in zygo1. Bars = 5 µm.