Figure 5.

Impact of the Loss of KfPPCK1 Activity on the Light/Dark Regulation of the Transcript Abundance of Central Circadian Clock Genes KfTOC1-1, KfTOC1-2, KfCCA1-1, KfCCA1-2, KfPRR7, and KfPRR37.

Mature leaves (leaf pair 6) were sampled from three biological replicates every 4 h across the 12-h-light/12-h-dark cycle, RNA isolated, and used for real-time RT-qPCR. TOC1-1 (A), TOC1-2 (B), CCA1-1 (C), CCA1-2 (D), PRR7 (E), and PRR37 (F). A thioesterase/thiol ester dehydrase-isomerase superfamily (KfTEDI) gene was amplified from the same cDNAs as a reference gene. Gene transcript abundance data represents the mean of three technical replicates for each of three biological replicates and was normalized to the reference gene (KfTEDI); error bars represent the se of the mean calculated for the three biological replicates. In all cases, plants were entrained under 12-h-light/12-h-dark cycles for 7 d prior to sampling. Black data are for the wild type, blue data rPPCK1-1, and red data rPPCK1-3.