Figure 6.

Impact of the Loss of KfPPCK1 Activity on Circadian Clock-Controlled Gene Transcript Abundance during Constant Light and Temperature Free-Running Conditions.

(A) Circadian rhythm of KfPPCK1 transcript abundance under constant LL conditions (100 µmol m⁻² s⁻¹ light at 15°C) for the wild type (black line) and rPPCK1-3 (red line).

(B) to (O) KfPPCK2 (B), KfPPCK3 (C), KfCCA1-1 (D), KfCCA1-2 (E), KfTOC1-1 (F), KfTOC1-2 (G), KfPRR7 (H), KfPRR37 (I), KfPRR9 (J), KfFKF1 (K), KfGIGANTEA (L), KfJMJD30 (M), KfLNK3-like (N), and KfCDF2 (O). Mature leaves (leaf pair 6) were sampled from three biological replicates every 4 h under constant conditions (100 µmol m⁻² s⁻¹ light at 15°C) for the wild type and rPPCK1-3. RNA was isolated and used for real-time RT-qPCR. A thioesterase/thiol ester dehydrase-isomerase superfamily gene (KfTEDI) was amplified as a reference gene from the same cDNAs. Gene transcript abundance data represent the mean of three technical replicates for each of three biological replicates and were normalized to the reference gene (KfTEDI); error bars represent the se of the mean calculated for the three biological replicates. In all cases, plants were entrained under 12-h-light/12-h-dark cycles prior to release into LL free-running conditions. Black data are for the wild type and red data rPPCK1-3.