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. 2017 Sep 25;29(10):2626–2643. doi: 10.1105/tpc.17.00370

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Figure 3. — MAC7 Promotes pri-miRNA Production.

(A) Determination of pri-miRNA levels in amiR-SUL and amiR-SUL mac7-1 inflorescences by RT-qPCR. The housekeeping gene IPP2 was included as a control. Expression levels were normalized to those of UBQ5 and compared with those in amiR-SUL (set to 1). Error bars indicate sd from three technical replicates. Asterisks indicate significant difference between amiR-SUL and amiR-SUL mac7-1 (t test, P < 0.05).

(B) Representative GUS staining images of pMIR167a:GUS and mac7-1 pMIR167a:GUS seedlings. The transcript levels of GUS and endogenous pri-miR167a in pMIR167a:GUS and mac7-1 pMIR167a:GUS seedlings were determined by RT-qPCR. Expression levels were normalized to those of UBQ5 and compared with those in pMIR167a:GUS (set to 1). Error bars indicate sd from three technical replicates. Asterisks, t test P < 0.05.

(C) Half-life measurements for pri-amiR-SUL, pri-miR167a, pri-miR172a, and EIF4A mRNA. Two-week-old amiR-SUL and amiR-SUL mac7-1 seedlings were treated with 0.6 mM cordycepin and harvested at various time points. RT-qPCR was performed to determine the levels of various pri-miRNAs and EIF4A mRNA. UBQ5 served as an internal control. Values at time 0 were set to 1. Error bars indicate sd from three technical replicates. Two biological replicates were performed and similar results were obtained.