Figure 7.

Stomatal Aperture Response and Rosette Growth of almt4 Knockout Lines Transformed with Full-Length ALMT4 S382A (S382A) and S382E (S382E).

(A) Representative images of wild-type, almt4, S382E, and S382A plants.

(B) Epidermal peels of wild-type (black), almt4 (blue), S382E (light green), and S382A (dark green) plants. Stomatal aperture was measured from dark-adapted plants, after a 2-h incubation under white light and subsequent 0.5- and 2-h incubations with 20 µM ABA. Data points are means of five different plants using 60 stomata per plant and significance of differences was tested between lines within one time point.

(C) Fresh weight of 6-week-old wild type, almt4, and two independent lines transformed with full-length ALMT4 S382A (S382A-1 and S382A-2) and S382E (S382E-1 and S382E-2), respectively (n = 6).

Data are means ± se. Significant differences were determined using ANOVA followed by multiple comparison (at least P ≤ 0.05) and are indicated by different letters.