Figure 1.

PGX3 Is Expressed in Multiple Tissues and Guard Cells.

(A) to (O) GUS staining of ProPGX3:GUS transgenic plants. Images show a 6-d-old etiolated hypocotyl (A), a 6-d-old light-grown seedling (B) with close-up views of a lateral root (C) and the root tip (D), a 3-week-old rosette leaf (E), stomatal guard cells in 3-week-old rosette leaves at different developmental stages with a meristemoid in (F), a guard mother cell in (G), guard cells with an initiating pore (H), young guard cells (I), and mature guard cells in (J). Additional images show a 6-week-old inflorescence in (K), a flower in (L), siliques in (M) and (N), and a mature dry seed in (O). Arrows in (A) and (B) indicate collet, which is the junction between the root and the hypocotyl. Arrows indicate a lateral root initiation site in (C) and a funiculus in (N). Bars = 1 mm in (A), (B), (K), (L), and (N), 0.5 mm in (C) and (D), 0.5 cm in (E), 10 µm in (F) to (J), 0.25 cm in (M), and 100 µm in (O).

(P) qPCR quantification of PGX3 expression in different tissues. The type and age of tissues used in qPCR experiments were consistent with those in GUS staining. ACT2 was used as an internal control, and PGX3 expression in hypocotyls was normalized to 1. Error bars are se and n = 3 biological replicates, with each biological replicate being an independent pool of tissues. For example, for 6-d-old etiolated hypocotyls, each biological replicate contained ∼40 dark-grown seedlings.