Figure 5.

PGX3 Modulates Stomatal Dynamics in Adult Plants.

(A) and (B) Average stomatal response to 1 µM FC-induced opening (A) or 50 µM ABA-induced closure (B) on the population level in 3- to 4-week-old Col, pgx3-1, PGX3 comp #3, and PGX3 OE #7 plants. Error bars are se (n ≥ 94 stomata per genotype per time point from three independent experiments; *P < 0.05, **P < 0.01, and ***P < 0.001, Student’s t test).

(C) and (D) Individual stomatal dynamics in 3- to 4-week-old Col controls, pgx3-1 mutants, and PGX3 OE #7 plants during 50 µM ABA-induced closure (n ≥ 30 stomata per genotype from at least four independent experiments). For each graph in (C), top, middle, and bottom lines correspond to the maximum, median, and minimum stomatal pore width values at each time point, respectively. Kymographs with quantifications in (D) were generated from the same set of images in Supplemental Movie 1. Double-headed arrows in each kymograph indicate stomatal pore width at the beginning or the end of ABA-induced closure.