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. 2017 Oct 3;29(10):2413–2432. doi: 10.1105/tpc.17.00568

Figure 8.

Figure 8.

Changes in PGX3 Expression Level Lead to Altered Total PG Activity and HG Molecular Mass.

(A) Total PG activity in vivo is significantly higher in PGX3 OE #7 plants. Total plant proteins were isolated from 33-d-old flowers. One unit of PG activity is the amount of enzyme releasing 1 µmol reducing end group per min per mg total protein at 30°C. Error bars are se, and lowercase letters represent significantly different groups (n ≥ 3 technical replicates per genotype per biological replicate, two biological replicates, and each biological replicate is an independent pool of flowers; P < 0.05, one-way ANOVA and Tukey test).

(B) Molecular mass analysis of CDTA-soluble pectins extracted from 34-d-old Col, pgx3-1, and PGX3 OE #7 rosette leaves. Molecular mass of the standards (β-amylase, 200 kD; BSA, 66 kD; carbonic anhydrase, 29 kD; and cytochrome c, 12.4 kD) is shown on the x axis at the top (n = 2 technical replicates per genotype per biological replicate, two biological replicates, and each biological replicate is an independent pool of rosette leaves).