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. Author manuscript; available in PMC: 2018 Jan 19.
Published in final edited form as: Leukemia. 2013 Sep 11;28(1):138–146. doi: 10.1038/leu.2013.260

Fig. 1. Multiple regulatory elements within the MEIS1 gene.

Fig. 1

A). Schematic map of enhancer elements in the MEIS1 Locus. Orthologous sequences in the MEIS1 locus are compared between human and mouse (Vista), with human sequence as the base. Numbered green bars (1 to 14) correspond to predicted enhancer elements conserved in multiple vertebrate species. The elements showing positive expression in the transgenic zebrafish assay are marked in red. Hg17: chr2:66,223,424-67,536,101 (corresponding to Hg19: 66311773-66680696)

B). Identification of enhancer activity by transgenic Zebrafish assay. The GFP reporter gene is under the control of a minimal promoter and the human sequence of putative enhancers. GFP reporter constructs were injected into fertilized zebrafish eggs. Representative transgenic zebrafish embryos (24 hpf) are shown, with the PLM area framed in blue, and ALM area circled in red. The percentage of positive embryos is marked in parentheses.